Microbiologist Food Safety 355/2013 Answer Key

Microbiologist

Post : Microbiologist Government Analytical Laboratories
Department : Food Safety
Category Code : 355/13
Qualification : Master Degree in Food and Industrial Microbiology
Question Paper Code : 75/2017/OL
Date of Test : 14-09-2017



  1. Which among the following organisms causes foodborne intoxication as a cause of food poisoning?
    • Staphylococcus aureus
    • Gonyaulax Catenella
    • Yersinia enterocolitica
    • Clavices Purpurea

  2. What is the optimum temperature for the growth of most of the psychrotrophs?
    • 15° C
    • 20° C
    • 1° C
    • 10° C

  3. Still birth in cattle and human beings is caused by the action of food poisoning that involves the presence of which of the bacteria?
    • Vibrio Cholarae
    • Listeria Monocytogenes
    • Campylobacter jejuni
    • Clostridium botulinum

  4. Name the antimicrobial ingredient present in clove
    • Cinnamaldehyde
    • Cineole
    • Thujone
    • Eugenol

  5. Select the Eh value limit, below which an environment is considered to be anaerobic in nature
    • 200 mV
    • -500 mV
    • -200 mV
    • -501 mV

  6. Name a mold like yeast among the following
    • Amylomyces
    • Endomycopsis
    • Candida
    • Pichia

  7. The maximum amount of alcohol content permissible in the case of table vines
    • 10%
    • 12%
    • 20%
    • 14%

  8. Name the fermented product from the juice of the cactus plant Agave
    • Pulque
    • Kemph
    • Sake
    • Kummis

  9. The flavouring compound with antibacterial activity that is present in the flower petals of the plant Hops that is used in the beer industry
    • Cadaverine and Putrescene
    • Flavons and Flavanones
    • Humulone and Lupulone
    • Thujone and ceneo

  10. How the personal hygiene in HACCP system in a meat processing plant is monitored?
    • Visual inspection
    • Hand swabs
    • Both Visual inspection and Hand swabs
    • None of these

  11. Which is the human organ that is affected by Veno-Occlusive disease?
    • Heart
    • Intestine
    • Liver
    • Kidneys

  12. Deoxynivalenol is grouped under the broad category of chemicals referred to as
    • Trichothecenes
    • Lectins
    • Terpenoids
    • Flavonoids

  13. Select the microaerophilic bacteria that causes food poisoning
    • Vibrio parahaemolyticus
    • Campylobacter jejuni
    • Bacillus cereus
    • Clostridium sp

  14. Mention the name of the scientist who first proposed the use of steel cans for the canning process
    • Francois Appert
    • Lazzaro Spallanzani
    • Peter Durand
    • Harry Russel

  15. Which of the following sequence accurately represent the Aw requirement in the decreasing order of demand for growth in food stuffs?
    • Spoilage bacteria > Spoilage yeast > Spoilage mold > Halophilic bacteria > Osmophilic yeast
    • Halophilic bacteria > Spoilage bacteria > Spoilage yeast > Spoilage mold > Osmophilic yeast
    • Spoilage bacteria > Spoilage yeast > Spoilage mold > Osmophilic yeast > Halophilic bacteria
    • Spoilage yeast > Spoilage bacteria > Spoilage mold > Halophilic bacteria > Osmophilic yeast

  16. Which among the following is not a Class I preservative?
    • Propionic acid
    • Dextrose
    • Honey
    • Sodium chloride

  17. Itai-Itai disease in Japan is caused by the toxicity of which of the following?
    • Arsenic
    • Copper
    • Mercury
    • Cadmium

  18. Name the toxic alkaloid present in Argemone mexicana which is a major adulterant in mustard
    • Thiocyanate
    • Dioxine
    • Sanguinarine
    • Trimethylamine

  19. During the production of beer making, in which of the following step the non soluble unfermentable carbohydrates from the cereals are hydrolyzed into soluble fermentable materials?
    • Mashing
    • Malting
    • Hop addition
    • Wort processing

  20. What is milk plasma?
    • Milk without lactose
    • Milk without casein
    • Milk without fat globules
    • Milk without whey

  21. Name the psychotropic bacteria belonging to enterobacteriaceae that causes enteric infection
    • Shigella Flexneri
    • Yersinia enterocolitica
    • Enterobacter fecalis
    • Escherichia Coli

  22. Which is the toxic protein present in ''Killer yeast'' that has adverse effect on beneficial yeast?
    • Mycocide
    • Bactocide
    • Zymocide
    • Saccharocide

  23. Which is the new taxonomical name given to the bottom fermenting yeast ''Saccharomyces carlsbergensis''?
    • Saccharomyces uvarum
    • Saccharomyces pastorianus
    • Saccharomyces udum
    • Saccharomyces lactis

  24. The scientist who first developed pure culture of yeast for the production of beer
    • Brefeld
    • Joseph Lister
    • Emil Hansen
    • Louis Pasteur

  25. Select the sequence of downstream processing involved in most of the production process of antibiotics
    • Filtration - Solvent Extraction - Crystallization - Drying
    • Solvent Extraction - Filtration - Crystallization - Drying
    • Filtration - Crystallization - Solvent Extraction - Drying
    • Crystallization - Solvent Extraction - Filtration - Drying

  26. Indonesian Tempeh is a processed food derived from
    • Rice
    • Soybean
    • Groundnut
    • Wheat

  27. Name the bacterial species with the presence of an active pyruvate carboxylase enzyme
    • Pseudomonas aeruginosa
    • Lactobacillus brevis
    • Streptococcus lactis
    • Zymomonas mobilis

  28. The enzyme responsible for the conversion of starch into fermentable sugars in the beer making process is
    • cellulase
    • amylase
    • glucanase
    • maltase

  29. The most important anaerobic bacterial species that causes meat putrefication is
    • Bacillus cereus
    • Clostridium perfringens
    • Clostridium tetani
    • Clostridium botulinum

  30. Name the neurotoxin that is involved in lathyrism
    • Trimethyl amine
    • Trimethyl amine oxide
    • Beta oxalyl amino alanine
    • Sanguinarine

  31. Select the temperature that can be tolerated by the emetic toxin of Bacillus cereus
    • 112°C
    • 85°C
    • 152°C
    • 126°C

  32. Which among the following is the causative agent for shell fish poisoning?
    • Gonyaulax Catenella
    • Vibrio Parahaemolyticus
    • Giardia lamblia
    • Salmonella typhimurium

  33. Select the critical limit of temperature as the CCP in the process steps of pasteurization in an HACCP plant for liquid milk
    • 138 ± 2°C
    • 55 ± 2°C
    • 77 ± 2°C
    • None of these

  34. VOD is caused by the toxin present in the seeds of which adulterant plant?
    • Crotalaria nanaburn
    • Panicum maximum
    • Argemone Mexicana
    • Arabidopsis Thaliana

  35. Which of the following condition the disease ''Hyperkeratosis'' is associated with?
    • Acute arsenic exposure
    • Chronic fluoride exposure
    • Acute fluoride exposure
    • Chronic arsenic exposure

  36. Pink colour formation in oysters is due to the infestation with which of the following organism?
    • Rhodotorula
    • Rhosdospirillum
    • Halobacterium
    • Serratia

  37. What do you mean by the term ''fugu''?
    • Algal poisoning
    • Shell fish poisoning
    • Algal blooming
    • Puffer fish poisoning

  38. Film forming yeast that causes the spoilage of wine is
    • Candida
    • Hansenula
    • Schizosaccharromyces
    • Albugo

  39. Which among the following is a heterofermentative lactic acid bacteria?
    • Lactococcus
    • Streptococcus
    • Leuconostoc
    • Pediococcus

  40. Select the factors that favour the growth of molds compared to bacteria in fruits
    • Low carbohydrate content and low pH
    • High carbohydrate content and low pH
    • High carbohydrate content and high pH
    • Low carbohydrate content and high pH

  41. Bacteriocins are used as a food preservative against which of the following?
    • Gram positive bacteria
    • Gram negative bacteria
    • Yeasts
    • Fungi

  42. Reuterine, an anti microbial substance produced by Lacto bacillus reuteri, that is used as a food preservative requires which of the following substance as a substrate for its synthesis?
    • Catechol
    • Tryptophan
    • Glycerol
    • Glucose

  43. Which among the following private enterprise contributed to the development of HACCP?
    • Nestle
    • Parle
    • Pillsbury
    • Cadbury

  44. Dye reduction test is used for assessing the quality of
    • Meat
    • Beer
    • Wine
    • Milk

  45. The destructive effect of ozone on bacteria was first noticed by
    • Kurkowitsch
    • Metchnikoff
    • Beijerinck
    • Paul Earhlich

  46. Which is the step that helps in determining the critical control points in an HACCP system?
    • Development of a flow chart
    • Development of a decision tree
    • Development of a flow diagram
    • Listing out of potential hazards

  47. Which is the mobile phase in gas chromatography?
    • Liquid
    • Solid
    • Gas
    • None of these

  48. 'Eyes' in Swiss cheese are formed as a result of
    • Entrapment of oxygen
    • By the action of rennin
    • Degradation of casein
    • Entrapment of CO2

  49. Which among the following is a fungal bioagent used for commercial production of α amylase?
    • Trichoderma viridae
    • Rhodotorula sp
    • Aspergillus oryzae
    • Aspergillus niger

  50. Which among the following is the actinobacteria used for the production of SCP?
    • Rhodococcus sp
    • Cellulomonas sp
    • Micrococcus sp
    • Corynebacterium sp

  51. In a continuous culture system which is the term that denotes the substrate concentration at which the specific growth rate is equal to half of the maximum specific growth rate?
    • Critical dilution constant
    • Specific consumption constant
    • Pasteur specific rate constant
    • Monod saturation constant

  52. Which of the following statement is not true with respect to HACCP?
    1. It is an end product test
    2. It is a preventive approach
    3. It is a continuous approach
    4. Corrective actions are taken at every stage
    • Statement 1
    • Statement 1 and 4
    • Statement 3
    • Statement 1 and 3

  53. To which of the following group Giardia lamblia belongs to
    • Fungus
    • Bacteria
    • Protozoa
    • Alga

  54. Select the pasteurization temperature used for ice cream mix
    • 71.1°C for 10 sec
    • 82.2°C for 20 sec
    • 65°C for 30 min
    • 71.1°C for 10 min

  55. In which of the following food stuff nitrates are used as chemical preservatives?
    • Meat
    • Egg
    • Vegetables
    • Milk

  56. A common mold that could be present in insufficiently canned fruit product is
    • Aspergillus niger
    • Byssochlamys fulva
    • Penicillium notatum
    • Phytophthora carotovora

  57. To which of the category the toxin Linamarin belongs to
    • Pyrrolizidene alkaloid
    • Carboxylic acid
    • Hydrated polyamine
    • Cyanogenic glycoside

  58. The first step in any HACCP programme is
    • To document the procedures
    • To conduct a hazard analysis
    • To establish critical limits
    • To determine critical control points

  59. Which is the most common detector in HPLC?
    • ECD
    • FID
    • UV/Vis
    • FTD

  60. Which is the starting substrate used for the production of white wine?
    • White grapes
    • De-skinned red/black grapes
    • None of these
    • Both White grapes and De-skinned red/black grapes

  61. Mention the factor responsible for affecting the digestibility of dietary protein in food
    • Pyruvate carboxylase
    • Ozxalates
    • Trypsin inhibitor
    • Goiterogens

  62. Select the hard cheese among the following
    • Cheddar
    • Camembert
    • Roquefort
    • Parmesan
    Question error

  63. Choose the enzymes that are not present in obligate anaerobic organisms
    • SOD
    • Catalase
    • SOD and catalase
    • Catalase and peroxidase

  64. Select the pH range at which the preservative action of sodium benzoate is the maximum
    • 5.6-7.0
    • 7.5-8.0
    • 2.5-4.0
    • 6.5-7.5

  65. Which is the symptom of spoilage caused by Cladosporium herbarum in meat?
    • Green patches
    • White spot
    • Whiskers
    • Black spot

  66. Which is the anaerobic bacterial species that produces a neurotoxin that is found associated with insufficient canning processes?
    • Bacillus cereus
    • Clostridium botulinum
    • Desulfovibrio sp
    • Desufatomaculum sp

  67. Which among the following is the spoilage symptom caused by Erwinia carotovora in vegetables?
    • Soft rot
    • Grey spot
    • Angular spot
    • Ropiness

  68. Detection of which of the compound in fish would reveal the spoilage occurrence?
    • TMAO
    • TMA
    • PUFA
    • TTX

  69. Paralytic shellfish poisoning is caused by
    • Saxitoxin by Gonyaulax catenella
    • Neurotoxin by dinoflagellates
    • Serotoxin by diatoms
    • None of these

  70. Which is the crhomatographic system that is used for analyzing samples with volatile activity?
    • TLC
    • Paper chromatography
    • GLC
    • Ion exchange chromatography

  71. Which is the most probable food poisoning organism that is associated with the complications arising out of the consumption of the Japanese food ''sushi''?
    • Camylobacter jejuni
    • Vibrio Cholarae
    • Pseudomonas aruginosa
    • Vibrio parahaemolyticus

  72. In a chemostat what is the dilution rate, if the flow rate is 50 ml per hr and the volume of the vessel is 500 ml?
    • 0.1 per hr
    • 10.0 per hr
    • 0.01 per hr
    • 25000 per hr

  73. Which of the fungus causes ergotism?
    • Claviceps purpurea
    • Rhizopus stolanifer
    • Aspergillus flavus
    • Fusarium oxysporum

  74. The starting material for the production of ''Rum'' is
    • Wheat
    • Barley
    • Grapes
    • Molasses

  75. Name the process of irradiation process in which viable, non-spore forming, pathogenic bacteria are eliminated from the food
    • Radicidation
    • Radurization
    • Radappertization
    • None of these

  76. Name the amino acid that is usually deficient in the single cell protein of microbial origin
    • L-tryptophan
    • L-methionine
    • L-arginine
    • L-cystine

  77. Which is the test that is used for assessing the efficiency of pasteurization of milk?
    • Hydrogenase test
    • Chemiluminescence test
    • Phosphatase test
    • Azide reduction test

  78. The number of days to lapse before the pesticide residue get dissipated is designated as
    • Dissipation period
    • Waiting period
    • Harvest duration
    • Lag period

  79. One gray in the radiation measurement is
    • 1000 rads
    • 100 erg
    • 100 rads
    • 1000 erg

  80. The causative agent for aflotoxicosis is
    • Fusarium oxysporum
    • Aspergillus flavus
    • Claviceps fusiformis
    • Rhizopus stolanifer

  81. The Radcliffe line is the international border between
    • India and China
    • India and Pakistan
    • India and Sri Lanka
    • India and Maldives

  82. The oldest fold mountain in India?
    • Nilgiri Mountain
    • Kanchenjunga
    • Vindhya hills
    • Aravalli Range

  83. Which of the following rivers originates from the Western Ghats near Mahabaleshwar Peak?
    • Krishna
    • Kaveri
    • Godavari
    • Sabarmati

  84. The battle of Plassey was fought between
    • Mir Qasim and East India Company
    • Shahalam and Robert Clive
    • Mirjafar and Robert Clive
    • Sirajuddaula and Robert Clive

  85. Who is known as ''The Father of Indian Renaissance''?
    • Rabindranath Tagore
    • Swami Vivekananda
    • Raja Ram Mohan Roy
    • Jawaharlal Nehru

  86. Poora Swaraj day was observed on
    • 8th August 1942
    • 26th January 1930
    • 22nd March 1940
    • 21st December 1928

  87. Who was the second President of Indian National Congress?
    • Dadabhai Naoroji
    • Mrs. Annie Besant
    • George Yule
    • Badruddin Tayyabji

  88. The 14 major Indian banks were nationalized during which five year plan?
    • Fifth plan
    • Ninth plan
    • Fourth plan
    • Second plan

  89. The second longest river in Kerala
    • Bharatha Puzha
    • Periyar
    • Pamba
    • Chalakudi

  90. Who was the founder of Thrissur City?
    • Kerala Varma
    • Achutha Menon
    • Raja Rama Varma
    • Panampilly Govinda Menon

  91. The First book in Malayalam literature questioning the cast system and untouchability?
    • Jathi Nirnayam
    • Adukkalayil ninnu arangathekku
    • Untouchables
    • Jathikummi

  92. The founder of the newspaper Swadeshabhimani?
    • Rama Krishna Pillai
    • Vakkom Moulavi
    • Sayyid Ahmed Khan
    • Madhavan Nair

  93. Ezhava Memorial was submitted to
    • Karthika Tirunal
    • Balarama Varma
    • Swathi Tirunal
    • Sree Moolam Tirunal

  94. Who was the founder of Athmavidya Sangam?
    • Vagbhatananda
    • Sree Narayana Guru
    • Chattampi Swami
    • Ayyankali

  95. Who was the founder of Samathwa Samajam?
    • Thycaud Ayya
    • Pandit Karuppan
    • Vaikunda Swami
    • Brahmananda Sivayogi

  96. The Brand Ambassador of Harithakeralam project
    • Manju Warrier
    • K.J. Yesudas
    • Mammootty
    • Mohanlal

  97. The Secretary General of SAARC
    • Arjun Bahadur Thapa
    • Ahmed Saleem
    • Sheel Sharma
    • Amjad Hussain B. Sial

  98. Which team wins the Ranji Trophy Final 2017?
    • Mumbai
    • Gujarat
    • Karnataka
    • Maharashtra

  99. Which launching vehicle of ISRO got the reward of 104 spacecraft in their desired orbits?
    • PSLV C : 34
    • PSLV C : 35
    • PSLV C : 37
    • PSLV C : 36

  100. Who is the latest recipient of Jnanpith Award?
    • Shankhe Ghosh
    • Raghuveer Chaudhari
    • Balachandra Nemade
    • Kedarnath Singh

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